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Season chuck roast on both sides with salt, pepper, and garlic seasoning
Sear roast on all sides in Instant Pot on sear setting or in a pan, then remove
Add chopped onions and garlic to the pot, cook until translucent
Add Worcestershire sauce and beef broth, deglazing the bottom of the pot
Stir in au jus packet and pepperoncini peppers with juice
Return beef to pot and close lid
Pressure cook for 2 hours (or slow cook in crockpot for 6-8 hours on low)
Allow natural pressure release
Remove meat and shred using a masher or two forks, return to pot with broth
Slice open sub rolls and spread mayo on buns
Layer shredded beef and provolone cheese on rolls
Toast buns in oven at 350-400°F or under broiler until golden and cheese melts
Serve immediately