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Preheat oven to 350°F and lightly grease a 9x13-inch baking dish with butter or nonstick spray.
Cook spaghetti until just al dente, usually 1-2 minutes less than package directions call for. Drain and return to pot.
Add spinach, chicken, and Alfredo sauce to pot and stir into spaghetti. Spread half of the mixture into prepared baking dish.
To a medium bowl, add the ricotta, sour cream, half of the mozzarella, egg, garlic, basil, and salt and pepper. Stir to combine.
Spread ricotta mixture over spaghetti and top with remaining spaghetti.
Top evenly with remaining mozzarella cheese and bake until golden and bubbly, 20-25 minutes.
Let stand 5 minutes before serving. Enjoy!